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ProductsBack/IL32A antibody (Biotin)
Western blot using GeneTexs HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
Western blot using GeneTexs HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
Western blot using GeneTexs HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.

IL32A antibody (Biotin)

GTX48653
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
TargetIL32
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL32A antibody (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000-1:10000. ELISA: 1:20000-1:100000. IHC: 1:1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Gene ID9235
  • Target name
    IL32
  • Target description
    interleukin 32
  • Target synonyms
    IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; tumor necrosis factor alpha-inducing factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24001
  • Protein Name
    Interleukin-32
  • Scientific Description
    IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (A03286). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL32 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.
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