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IHC-P analysis of formaldehyde fixed Human Lymph Node using GTX31317 IL33 antibody. Antigen retreival : Heat mediation with a citrate buffer (pH6) Dilution : 2.5microg/mL
IHC-P analysis of formaldehyde fixed Human Lymph Node using GTX31317 IL33 antibody. Antigen retreival : Heat mediation with a citrate buffer (pH6) Dilution : 2.5microg/mL
IHC-P analysis of formaldehyde fixed Human Lymph Node using GTX31317 IL33 antibody. Antigen retreival : Heat mediation with a citrate buffer (pH6) Dilution : 2.5microg/mL

IL33 antibody

GTX31317
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetIL33
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL33 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2 microg/mL. IHC-P: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID90865
  • Target name
    IL33
  • Target description
    interleukin 33
  • Target synonyms
    C9orf26; DVS27; DVS27-related protein; IL1F11; interleukin-1 family member 11; interleukin-33; NFEHEV; NF-HEV; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95760
  • Protein Name
    Interleukin-33
  • Scientific Description
    IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition.
    Read more

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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL33 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 18KD. The expected band size for IL33 is at 18KD.
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