Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 microg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.
Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 microg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.
IL6 antibody
GTX26672
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetIL6
Overview
- SupplierGeneTex
- Product NameIL6 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. IHC-P: 1:1:100-1:200. ELISA: 1:500-1:2000. Neutralizing/Inhibition: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration80 mg/ml
- ConjugateUnconjugated
- Gene ID3569
- Target nameIL6
- Target descriptioninterleukin 6
- Target synonymsB-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6
- HostRabbit
- IsotypeIgG
- Protein IDP05231
- Protein NameInterleukin-6
- Scientific DescriptionInterleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important asIL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Rib soft fixation produces better analgesic effects and is associated with cytokine changes within the spinal cord in a rat rib fracture model. Liu YY et al., 2019 Jan-Dec, Mol PainRead more
- Importance of IL-6, MMP-1, IGF-1, and BAX Levels in Lumbar Herniated Disks and Posterior Longitudinal Ligament in Patients with Sciatic Pain. Dagistan Y et al., 2015 Dec, World NeurosurgRead more
- MAPK signaling in the quadriceps of patients with chronic obstructive pulmonary disease. Lemire BB et al., 2012 Jul, J Appl Physiol (1985)Read more