Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse IL-6 Protein (169.49-2.65 nM). Coated protein was probed with IL6 antibody [HL2320] (GTX638483) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
![Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (168.63-2.63 nM). Coated protein was probed with IL6 antibody [HL2320] (GTX638483) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638483/GTX638483_T-45005_20241108_ELISA_Indirect_24120522_870.webp "Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (168.63-2.63 nM). Coated protein was probed with IL6 antibody [HL2320] (GTX638483) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2320] (GTX638483) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX638483/GTX638483_45614_20250117_ELISA_PAIR_25020422_446.webp "Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2320] (GTX638483) (1 μg/mL)")
![IL6 antibody [HL2320] detects IL6 protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: IL6 stained by IL6 antibody [HL2320] (GTX638483) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638483/GTX638483_45614_20250627_ICC_IF_treatment_TPA_LPS_BFA_25071023_537.webp "IL6 antibody [HL2320] detects IL6 protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: IL6 stained by IL6 antibody [HL2320] (GTX638483) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).")
Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse IL-6 Protein (169.49-2.65 nM). Coated protein was probed with IL6 antibody [HL2320] (GTX638483) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
IL6 antibody [HL2320]
GTX638483
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIL6
Overview
- SupplierGeneTex
- Product NameIL6 antibody [HL2320]
- Delivery Days Customer7
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2320
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3569
- Target nameIL6
- Target descriptioninterleukin 6
- Target synonymsBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, interleukin-6, B-cell differentiation factor, B-cell stimulatory factor 2, CTL differentiation factor, hybridoma growth factor, interferon beta-2, interleukin BSF-2
- HostRabbit
- IsotypeIgG
- Protein IDP05231
- Protein NameInterleukin-6
- Scientific DescriptionThis gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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