![Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody. Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_T-45047_20230609_ELISA_Indirect_23061320_266.webp)
Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
IL6 antibody [HL2389]
GTX638598
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIL6
Overview
- SupplierGeneTex
- Product NameIL6 antibody [HL2389]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2389
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3569
- Target nameIL6
- Target descriptioninterleukin 6
- Target synonymsBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, interleukin-6, B-cell differentiation factor, B-cell stimulatory factor 2, CTL differentiation factor, hybridoma growth factor, interferon beta-2, interleukin BSF-2
- HostRabbit
- IsotypeIgG
- Protein IDP05231
- Protein NameInterleukin-6
- Scientific DescriptionThis gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20230721_WB_treatment_TPA_LPS_BFA_23072519_658.webp)
![Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL) Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20230721_ELISA_PAIR_2_23072519_926.webp)
![Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL) Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20230721_ELISA_PAIR_23072519_428.webp)
![Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20230721_WB_Ag_competitor_watermark_23072519_695.webp)
![Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20230804_WB_treatment_TPA_LPS_BFA_23080901_185.webp)
![IL6 antibody [HL2389] detects IL6 protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920). IL6 antibody [HL2389] detects IL6 protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638598/GTX638598_45110_20250627_ICC_IF_treatment_TPA_LPS_BFA_25071023_308.webp)


![Sandwich ELISA analysis of human IL-6 protein using GTX02999 IL6 antibody [13A5] as coating antibody and GTX03001-02 IL6 antibody [39C3] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02999/GTX02999_20210507_ELISA_w_23053123_562.webp)
![Sandwich ELISA analysis of human IL-6 protein using GTX02999 IL6 antibody [13A5] as coating antibody and GTX03001-02 IL6 antibody [39C3] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03001-02/GTX03001-02_20210507_ELISA_w_23053123_512.webp)
![Sandwich ELISA analysis of human IL-6 protein using GTX02999 IL6 antibody [13A5] as coating antibody and GTX03001-02 IL6 antibody [39C3] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03095/GTX03095_20210507_ELISA_w_23053123_347.webp)
![Sandwich ELISA analysis of mouse IL-6 protein using GTX03002 IL6 antibody [MT26B2] as coating antibody and GTX03000-02 IL6 antibody [32C11] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03096/GTX03096_20210507_ELISA_w_23053123_910.webp)

