
IHC analysis of colchicine injected mouse brain (cingulate cortex) tissue using IL6 antibody at a concentration of 0.5 microg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.
IL6 antibody
GTX31178
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
TargetIl6
Overview
- SupplierGeneTex
- Product NameIL6 antibody
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 microg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 microg/ml. For IHC-Fr: Use at a concentration of 0.5 microg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Mouse IL-6 (3 pg/ml), a concentration of 0.24-0.36 microg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID16193
- Target nameIl6
- Target descriptioninterleukin 6
- Target synonymsB-cell hybridoma growth factor; Il; Il-6; interleukin HP-1; interleukin-6
- HostRabbit
- IsotypeIgG
- Protein IDP08505
- Protein NameInterleukin-6
- Scientific DescriptionThis gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Torenia concolor Lindley var. formosana Yamazaki extracts improve inflammatory response and lipid accumulation via PPARs activation. Liang YC et al., 2017 Sep, Biomedicine (Taipei)Read more
- Gpr110 deficiency decelerates carcinogen-induced hepatocarcinogenesis via activation of the IL-6/STAT3 pathway. Ma B et al., 2017, Am J Cancer ResRead more
- Supplementation of Magnolol Attenuates Skeletal Muscle Atrophy in Bladder Cancer-Bearing Mice Undergoing Chemotherapy via Suppression of FoxO3 Activation and Induction of IGF-1. Chen MC et al., 2015, PLoS OneRead more