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IL6 / Interleukin 6 Antibody (Biotin)

LS-C26506
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetIL6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL6 / Interleukin 6 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA: This antibody has been used as a solution-phase antibody at 2 microg/ml ina two-site ELISA with unconjugated IL-6 antibody (Cat. No. LS-C26496). Typicallly,the ELISA has a working range of 0-10000 pg/ml and sensitivity 5 pg/ml withthe appropriate standard. Recommended sample volumes are 25-200 microl.Recovery of IL-6 added to human plasma is typically >85% in the ELISA. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA ELISA: This antibody has been used as a solution-phase antibody at 2 µg/ml ina two-site ELISA with unconjugated IL-6 antibody (Cat. No. LS-C26496). Typicallly,the ELISA has a working range of 0-10000 pg/ml and sensitivity 5 pg/ml withthe appropriate standard. Recommended sample volumes are 25-200 µl.Recovery of IL-6 added to human plasma is typically >85% in the ELISA. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID3569
  • Target name
    IL6
  • Target description
    interleukin 6
  • Target synonyms
    B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL6 protein 10 ng, Lane 2: recombinant human IL6 protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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