
Sample (30 μg of whole cell lysate) A: zebrafish heart 10% SDS PAGE GTX107443 diluted at 1:1000
ILK antibody [N1C1-2]
GTX107443
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetILK
Overview
- SupplierGeneTex
- Product NameILK antibody [N1C1-2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3611
- Target nameILK
- Target descriptionintegrin linked kinase
- Target synonymsHEL-S-28, ILK-1, ILK-2, P59, p59ILK, scaffold protein ILK, 59 kDa serine/threonine-protein kinase, beta-integrin-linked kinase, epididymis secretory protein Li 28, inactive integrin-linked kinase, integrin-linked kinase-2, integrin-linked protein kinase
- HostRabbit
- IsotypeIgG
- Protein IDQ13418
- Protein NameScaffold protein ILK
- Scientific DescriptionTransduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1-2] (GTX107443) at 1:300 dilution. Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1-2] (GTX107443) at 1:300 dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107443/GTX107443_40037_IHC_Z_22111423_360.webp)
![Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1-2] (GTX107443) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1-2] (GTX107443) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107443/GTX107443_40037_20170330_WB_KO_watermark_w_23060120_405.webp)





![Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)](https://www.genetex.com/upload/website/prouct_img/normal/GTX101691/GTX101691_39876_IHC-Wm_Z_22111422_336.webp)
![FACS analysis of Jurkat cells using GTX60645 ILK antibody [3A9]. Green : ILK Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60645/GTX60645_20170912_FACS_w_23061123_148.webp)