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SH-SY5Y lysates (20ug) probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
SH-SY5Y lysates (20ug) probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
SH-SY5Y lysates (20ug) probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

INA (7T2) Monoclonal Antibody

BSM-51074M
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetINA
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Overview

  • Supplier
    Bioss
  • Product Name
    INA (7T2) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7T2
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID9118
  • Target name
    INA
  • Target description
    internexin neuronal intermediate filament protein alpha
  • Target synonyms
    NEF5, NF-66, NF66, TXBP-1, alpha-internexin, 66 kDa neurofilament protein, alpha-Inx, neurofilament 5 (66kD), neurofilament-66, tax-binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16352
  • Protein Name
    Alpha-internexin
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Internexin/INA antigen affinity purified polyclonal antibody (Catalog # A03756-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Internexin/INA at approximately 66 kDa. The expected band size for Alpha Internexin/INA is at 66 kDa.
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