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ProductsBack/INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI1A4]
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IDO1 plasmid (RC206592, hIDO1), human IDO2 plasmid (RC223337, hIDO2) using anti-IDO2 antibody TA501378(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IDO1 plasmid (RC206592, hIDO1), human IDO2 plasmid (RC223337, hIDO2) using anti-IDO2 antibody TA501378(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IDO1 plasmid (RC206592, hIDO1), human IDO2 plasmid (RC223337, hIDO2) using anti-IDO2 antibody TA501378(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI1A4]

CF501378
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetIDO2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI1A4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1A4
  • Gene ID169355
  • Target name
    IDO2
  • Target description
    indoleamine 2,3-dioxygenase 2
  • Target synonyms
    INDOL1, indoleamine 2,3-dioxygenase 2, indoleamine 2,3-dioxygenase-like 1 protein, indoleamine 2,3-dioxygenase-like protein 1, indoleamine-pyrrole 2,3 dioxygenase-like 1, indoleamine-pyrrole 2,3-dioxygenase-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ6ZQW0
  • Protein Name
    Indoleamine 2,3-dioxygenase 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) IDO2 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates (positive control), Lane 2: human placenta tissue lysates (positive control), Lane 3: human Caco-2 whole cell lysates (positive control), Lane 4: human PC-3 whole cell lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
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