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WB analysis of HepG2 lysate using GTX89918 ING2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HepG2 lysate using GTX89918 ING2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HepG2 lysate using GTX89918 ING2 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer

ING2 antibody, C-term

GTX89918
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetING2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ING2 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3622
  • Target name
    ING2
  • Target description
    inhibitor of growth family member 2
  • Target synonyms
    ING1L; ING1Lp; inhibitor of growth 1-like protein; inhibitor of growth protein 2; p32; p33ING2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9H160
  • Protein Name
    Inhibitor of growth protein 2
  • Scientific Description
    This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ING2 using anti-ING2 antibody (A04290-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse EL-4 whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING2 antigen affinity purified polyclonal antibody (Catalog # A04290-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING2 at approximately 37 kDa. The expected band size for ING2 is at 33 kDa.
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