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Western Blot analysis of Lovo cell,Human liver cancer tissue and Mouse kidney tissue using ING2 Polyclonal Antibody at dilution of 1:425
Western Blot analysis of Lovo cell,Human liver cancer tissue and Mouse kidney tissue using ING2 Polyclonal Antibody at dilution of 1:425
Western Blot analysis of Lovo cell,Human liver cancer tissue and Mouse kidney tissue using ING2 Polyclonal Antibody at dilution of 1:425

ING2 Polyclonal Antibody

E-AB-14158
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ING2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ING2 using anti-ING2 antibody (A04290-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse EL-4 whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING2 antigen affinity purified polyclonal antibody (Catalog # A04290-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING2 at approximately 37 kDa. The expected band size for ING2 is at 33 kDa.
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