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INHBA / Inhibin Beta A Antibody

LS-C405455
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetINHBA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    INHBA / Inhibin Beta A Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3624
  • Target name
    INHBA
  • Target description
    inhibin subunit beta A
  • Target synonyms
    EDF, FRP, inhibin beta A chain, FSH-releasing protein, Inhibin, beta-1, activin beta-A chain, erythroid differentiation factor, erythroid differentiation protein, follicle-stimulating hormone-releasing protein, inhibin beta A subunit, inhibin, beta A (activin A, activin AB alpha polypeptide)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Inhibin beta A/INHBA using anti-Inhibin beta A/INHBA antibody (M06777). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Inhibin beta A/INHBA antigen affinity purified monoclonal antibody (M06777) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Inhibin beta A/INHBA at approximately 43 kDa. The expected band size for Inhibin beta A/INHBA is at 47 kDa.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody (GTX108405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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