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Immunohistochemical analysis of paraffin-embedded human lung cancer, using Insulin Receptor beta antibody (GTX111314) at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Insulin Receptor beta antibody (GTX111314) at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Insulin Receptor beta antibody (GTX111314) at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

Insulin Receptor beta antibody

GTX111314
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetINSR
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Overview

  • Supplier
    GeneTex
  • Product Name
    Insulin Receptor beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.67 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3643
  • Target name
    INSR
  • Target description
    insulin receptor
  • Target synonyms
    CD220, HHF5, insulin receptor, IR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06213
  • Protein Name
    Insulin receptor
  • Scientific Description
    After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Insulin Receptor/INSR using anti-Insulin Receptor/INSR antibody (A00447-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor/INSR antigen affinity purified polyclonal antibody (Catalog # A00447-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor/INSR at approximately 156 kDa. The expected band size for Insulin Receptor/INSR is at 156 kDa.
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