WB (peptide competition) analysis of CHO-T cells over-expressing the human insulin receptor stimulated with 100 nM insulin for 10 min at 37oC (Lane 2-5) using GTX25680 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphotyrosine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of CHO-T cells over-expressing the human insulin receptor stimulated with 100 nM insulin for 10 min at 37oC (Lane 2-5) using GTX25680 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphotyrosine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody
GTX25680
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetINSR
Overview
- SupplierGeneTex
- Product NameIGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody is raised against a synthetic peptide around phosphorylated Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2), which is identical to the sequence around phosphorylated Tyr1135 / Tyr1136 of IGF1R (signal peptide
- Application Supplier NoteICC/IF: 1:100-1:500. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3643
- Target nameINSR
- Target descriptioninsulin receptor
- Target synonymsCD220; HHF5; insulin receptor; IR
- HostRabbit
- IsotypeIgG
- Protein IDP06213
- Protein NameInsulin receptor
- Scientific DescriptionThis gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2. Huang TW et al., 2019 Oct 1, Acta BiomaterRead more