Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.
![Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 microg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 microg/ml.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15624/GTX15624_21_ELISA_w_23060620_324.webp "Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 microg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 microg/ml.")
Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.
Interferon gamma antibody [2G1]
GTX15624
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIFNG
Overview
- SupplierGeneTex
- Product NameInterferon gamma antibody [2G1] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2G1
- Concentration1.04 mg/ml
- ConjugateUnconjugated
- Gene ID3458
- Target nameIFNG
- Target descriptioninterferon gamma
- Target synonymsIFG; IFI; IFN-gamma; IMD69; immune interferon; interferon gamma
- HostMouse
- IsotypeIgG1
- Protein IDP01579
- Protein NameInterferon gamma
- Scientific DescriptionThis gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Withasteroid B from D. metel L. regulates immune responses by modulating the JAK/STAT pathway and the IL-17+ RORgammat+ /IL-10+ FoxP3+ ratio. Su Y et al., 2017 Oct, Clin Exp ImmunolRead more
- Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis. Fang S et al., 2016 Mar, Neuromolecular MedRead more
Datasheet
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