Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 μg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 μg/ml.
![Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15624/GTX15624_20_ELISA_w_23060620_585.webp "Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.")
Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 μg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 μg/ml.
Interferon gamma antibody [2G1]
GTX15624
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIFNG
Overview
- SupplierGeneTex
- Product NameInterferon gamma antibody [2G1]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2G1
- Concentration1.04 mg/ml
- ConjugateUnconjugated
- Gene ID3458
- Target nameIFNG
- Target descriptioninterferon gamma
- Target synonymsIFG, IFI, IMD69, interferon gamma, IFN-gamma, immune interferon
- HostMouse
- IsotypeIgG1
- Protein IDP01579
- Protein NameInterferon gamma
- Scientific DescriptionThis gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Su Y, Wang Q, Yang B, et al. Withasteroid B from D. metel L. regulates immune responses by modulating the JAK/STAT pathway and the IL-17(+) RORγt(+) /IL-10(+) FoxP3(+) ratio. Clin Exp Immunol. 2017,190(1):40-53. doi: 10.1111/cei.12998Read this paper
- Fang S, Meng X, Zhang Z, et al. Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis. Neuromolecular Med. 2016,18(1):134-45. doi: 10.1007/s12017-016-8383-0Read this paper
Datasheet
Related products
Product group Antibodies
Anti-IFN-y [G-23], Mouse IgG1, kappaAB04292-1.1
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetIFNG
- SizePrice
Product group Antibodies
IFNG Polyclonal AntibodyCAC13774
ApplicationsWestern Blot, ELISA
TargetIFNG
- SizePrice
Product group Antibodies
References
IFN gamma Polyclonal AntibodyBS-0481R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIFNG
- SizePrice
Product group Antibodies
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIFNG
- SizePrice
Product group Antibodies
Anti-IFN-gamma AntibodyA98568
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
- SizePrice
![WB analysis of indicated amount of human IFN Gamma recombinant protein using GTX15750 Interferon gamma antibody [B133.5]. Dilution : 2 μg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15750/GTX15750_1523_WB_w_23060620_450.webp)
Product group Antibodies
References
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
ReactivityHuman, Mouse
TargetIFNG
- SizePrice
![Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15751/GTX15751_54_ELISA_w_23060620_349.webp)
Product group Antibodies
References
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
ReactivityHuman, Mouse
TargetIFNG
- SizePrice