Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.
![Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 microg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 microg/ml.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15751/GTX15751_55_ELISA_w_23060620_120.webp "Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 microg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 microg/ml.")
Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.
Interferon gamma antibody [B133.5] (Biotin)
GTX15751
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
TargetIFNG
Overview
- SupplierGeneTex
- Product NameInterferon gamma antibody [B133.5] (Biotin) - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDB133.5
- Concentration0.5 mg/ml
- ConjugateBiotin
- Gene ID3458
- Target nameIFNG
- Target descriptioninterferon gamma
- Target synonymsIFG, IFI, IMD69, interferon gamma, IFN-gamma, immune interferon
- HostMouse
- IsotypeIgG1
- Protein IDP01579
- Protein NameInterferon gamma
- Scientific DescriptionThis gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Neutralizing Activity of Anti-interferon-gamma Autoantibodies in Adult-Onset Immunodeficiency Is Associated With Their Binding Domains.Read more
- Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA.Read more
- Expression of immune modulator cytokines in human fulminant amoebic colitis.Read more
- Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes.Read more
- Digital quantification of the enzyme-linked immunospot (ELISPOT).Read more
Datasheet
Related products
Product group Antibodies
IFNG AntibodyCSB-PA003000
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIFNG
- SizePrice
Product group Antibodies
References
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetIFNG
- SizePrice
![Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15624/GTX15624_20_ELISA_w_23060620_585.webp)
Product group Antibodies
References
Interferon gamma antibody [2G1]GTX15624
ApplicationsWestern Blot, ELISA
TargetIFNG
- SizePrice
![FACS analysis of permeabilized, PHA-M treated Jurkat cells using GTX15750 Interferon gamma antibody [B133.5]. Fixation : 4% formaldehyde Dilution : 1:100 with IC permeabilization buffer for 30 min on ice](https://www.genetex.com/upload/website/prouct_img/normal/GTX15750/GTX15750_106_FACS_w_23060620_791.webp)
Product group Antibodies
References
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetIFNG
- SizePrice
Product group Antibodies
ApplicationsELISA
TargetIFNG
- SizePrice
Product group Antibodies
Interferon gamma antibody [G-23]GTX27415
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetIFNG
- SizePrice
Product group Antibodies
Interferon gamma antibodyGTX10741
ApplicationsWestern Blot, Neutralisation/Blocking
TargetIFNG
- SizePrice
Product group Antibodies
Interferon gamma antibodyGTX11516
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetIFNG
- SizePrice
![FACS (intracellular staining) analysis of PHA-activated human PBMC using GTX00484-06 Interferon gamma antibody [4S.B3] (FITC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX00484-06/GTX00484-06_20191025_AP_006_25_w_23053121_403.webp)
Product group Antibodies
Interferon gamma antibody [4S.B3] (FITC)GTX00484-06
ApplicationsFlow Cytometry
TargetIFNG
- SizePrice
![FACS (intracellular staining) analysis of PHA-activated human PBMC using GTX00484-07 Interferon gamma antibody [4S.B3] (APC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX00484-07/GTX00484-07_20191025_AP_006_26_w_23053121_316.webp)
Product group Antibodies
Interferon gamma antibody [4S.B3] (APC)GTX00484-07
ApplicationsFlow Cytometry
TargetIFNG
- SizePrice