FACS analysis of permeabilized, PHA-M treated Jurkat cells using GTX15750 Interferon gamma antibody [B133.5]. Fixation : 4% formaldehyde Dilution : 1:100 with IC permeabilization buffer for 30 min on ice
![WB analysis of indicated amount of human IFN Gamma recombinant protein using GTX15750 Interferon gamma antibody [B133.5]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15750/GTX15750_1523_WB_w_23060620_450.webp "WB analysis of indicated amount of human IFN Gamma recombinant protein using GTX15750 Interferon gamma antibody [B133.5]. Dilution : 2 microg/ml")
FACS analysis of permeabilized, PHA-M treated Jurkat cells using GTX15750 Interferon gamma antibody [B133.5]. Fixation : 4% formaldehyde Dilution : 1:100 with IC permeabilization buffer for 30 min on ice
Interferon gamma antibody [B133.5]
GTX15750
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman, Mouse
TargetIFNG
Overview
- SupplierGeneTex
- Product NameInterferon gamma antibody [B133.5]
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/ml. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDB133.5
- Concentration0.98 mg/ml
- ConjugateUnconjugated
- Gene ID3458
- Target nameIFNG
- Target descriptioninterferon gamma
- Target synonymsIFG, IFI, IMD69, interferon gamma, IFN-gamma, immune interferon
- HostMouse
- IsotypeIgG1
- Protein IDP01579
- Protein NameInterferon gamma
- Scientific DescriptionThis gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yasamut U, Thongkum W, Moonmuang S, et al. Neutralizing Activity of Anti-interferon-γ Autoantibodies in Adult-Onset Immunodeficiency Is Associated With Their Binding Domains. Front Immunol. 2019,10:1905. doi: 10.3389/fimmu.2019.01905Read this paper
- Kimoto M, Shermane Lim YW, Hirao I. Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA. Nucleic Acids Res. 2019,47(16):8362-8374. doi: 10.1093/nar/gkz688Read this paper
- Sierra-Puente RE, Campos-Rodríguez R, Jarillo-Luna RA, et al. Expression of immune modulator cytokines in human fulminant amoebic colitis. Parasite Immunol. 2009,31(7):384-91. doi: 10.1111/j.1365-3024.2009.01118.xRead this paper
- Hartig CV, Haller GW, Sachs DH, et al. Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes. J Immunol. 2000,164(5):2790-6.Read this paper
- Vaquerano JE, Peng M, Chang JW, et al. Digital quantification of the enzyme-linked immunospot (ELISPOT). Biotechniques. 1998,25(5):830-4, 836.Read this paper
Datasheet
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