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Interleukin 18 (IL18) Monoclonal Antibody

MBS340906
MyBioSource
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Product group Antibodies
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0.1 mg
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Overview

  • Supplier
    MyBioSource
  • Product Name
    Interleukin 18 (IL18) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA081015(IL18 Antibody) at dilution 1/120, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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