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Involucrin antibody [SY5]

GTX72415
GeneTex
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIVL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Involucrin antibody [SY5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ELISA (For coating, order Ab without BSA).
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SY5
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3713
  • Target name
    IVL
  • Target description
    involucrin
  • Target synonyms
    involucrin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07476
  • Protein Name
    Involucrin
  • Scientific Description
    Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Li Z, Han S, Wang X, et al. Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium. Stem Cell Res Ther. 2015,6(1):17. doi: 10.1186/s13287-015-0008-2
    Read this paper
  • Suárez-Fariñas M, Tintle SJ, Shemer A, et al. Nonlesional atopic dermatitis skin is characterized by broad terminal differentiation defects and variable immune abnormalities. J Allergy Clin Immunol. 2011,127(4):954-64.e1-4. doi: 10.1016/j.jaci.2010.12.1124
    Read this paper
  • Biedermann T, Pontiggia L, Böttcher-Haberzeth S, et al. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010,130(8):1996-2009. doi: 10.1038/jid.2010.83
    Read this paper

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Figure 1. Western blot analysis of Involucrin/IVL using anti-Involucrin/IVL antibody (PB9712). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Involucrin/IVL antigen affinity purified polyclonal antibody (Catalog # PB9712) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Involucrin/IVL at approximately 120 kDa. The expected band size for Involucrin/IVL is at 68 kDa.
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