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ProductsBack/IP3KC antibody [N3C2], Internal

IP3KC antibody [N3C2], Internal

GTX104192
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetITPKC
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Overview

  • Supplier
    GeneTex
  • Product Name
    IP3KC antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.84 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80271
  • Target name
    ITPKC
  • Target description
    inositol-trisphosphate 3-kinase C
  • Target synonyms
    inositol 1,4,5-trisphosphate 3-kinase C; inositol-trisphosphate 3-kinase C; InsP 3 kinase C; insP 3-kinase C; IP3 3-kinase C; IP3-3KC; IP3K C; IP3KC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DU7
  • Protein Name
    Inositol-trisphosphate 3-kinase C
  • Scientific Description
    Inositol 1,4,5-trisphosphate 3-kinase (ITPK) catalyzes the phosphorylation of Ins(1,4,5)P3 to Ins(1,3,4,5)P4, both of which are modulators of calcium homeostasis. ITPK isoforms include ITPKA (MIM 147521), ITPKB (MIM 147522), and ITPKC, all of which contain a conserved catalytic unit in their C termini, but have unique N-terminal sequences and tissue distributions.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITPKC using anti-ITPKC antibody (A07845-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPKC antigen affinity purified polyclonal antibody (Catalog # A07845-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPKC at approximately 100 kDa. The expected band size for ITPKC is at 75 kDa.
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