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Western Blot analysis of HT29 cells using InsP 3-kinase C Polyclonal Antibody
Western Blot analysis of HT29 cells using InsP 3-kinase C Polyclonal Antibody
Western Blot analysis of HT29 cells using InsP 3-kinase C Polyclonal Antibody

ITPKC Antibody

CSB-PA003041
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITPKC
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITPKC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80271
  • Target name
    ITPKC
  • Target description
    inositol-trisphosphate 3-kinase C
  • Target synonyms
    IP3-3KC, IP3KC, inositol-trisphosphate 3-kinase C, IP3 3-kinase C, IP3K C, InsP 3 kinase C, inositol 1,4,5-trisphosphate 3-kinase C, insP 3-kinase C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DU7
  • Protein Name
    Inositol-trisphosphate 3-kinase C
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITPKC using anti-ITPKC antibody (A07845-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPKC antigen affinity purified polyclonal antibody (Catalog # A07845-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPKC at approximately 100 kDa. The expected band size for ITPKC is at 75 kDa.
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