ICC/IF analysis of COS7 cells transiently transfected with IRF3 plasmid using GTX84288 IRF3 antibody [4D4].
![IHC-P analysis of human pancreas tissue using GTX84288 IRF3 antibody [4D4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84288/GTX84288_2569_IHC-P_w_23061420_847.webp "IHC-P analysis of human pancreas tissue using GTX84288 IRF3 antibody [4D4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of HEK293T cells transfected with either IRF3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84288 IRF3 antibody [4D4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84288/GTX84288_343_FACS_w_23061420_828.webp "FACS analysis of HEK293T cells transfected with either IRF3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84288 IRF3 antibody [4D4].")
![WB analysis of HEK293T cells transfected with IRF3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84288 IRF3 antibody [4D4]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84288/GTX84288_4241_WB_w_23061420_164.webp "WB analysis of HEK293T cells transfected with IRF3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84288 IRF3 antibody [4D4]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84288 IRF3 antibody [4D4]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84288/GTX84288_4242_WB_w_23061420_424.webp "WB analysis of various cell lines using GTX84288 IRF3 antibody [4D4]. Loading : 35 ug per lane")
ICC/IF analysis of COS7 cells transiently transfected with IRF3 plasmid using GTX84288 IRF3 antibody [4D4].
IRF3 antibody [4D4]
GTX84288
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIRF3
Overview
- SupplierGeneTex
- Product NameIRF3 antibody [4D4] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D4
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3661
- Target nameIRF3
- Target descriptioninterferon regulatory factor 3
- Target synonymsIIAE7; interferon regulatory factor 3
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionMediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203