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ProductsBack/ITGA2 / CD49b Antibody (clone DX5, PE, Cy7)

ITGA2 / CD49b Antibody (clone DX5, PE, Cy7)

LS-C812209
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA2 / CD49b Antibody (clone DX5, PE, Cy7)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DX5
  • Concentration
    0.2 mg/ml
  • Conjugate
    Cy7, RPE
  • Estimated Purity
    ...
  • Gene ID3673
  • Target name
    ITGA2
  • Target description
    integrin subunit alpha 2
  • Target synonyms
    alpha 2 subunit of VLA-2 receptor; BR; CD49 antigen-like family member B; CD49B; collagen receptor; GPIa; HPA-5; human platelet alloantigen system 5; integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2; VLAA2
  • Host
    Rat
  • Isotype
    IgM
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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