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ITGA2 / CD49b Antibody

LS-C405885
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITGA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA2 / CD49b Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3673
  • Target name
    ITGA2
  • Target description
    integrin subunit alpha 2
  • Target synonyms
    BR, CD49B, FMAIT3, GPIa, HPA-5, VLA-2, VLAA2, integrin alpha-2, CD49 antigen-like family member B, alpha 2 integrin, alpha 2 subunit of VLA-2 receptor, collagen receptor, glycoprotein Ia, human platelet alloantigen 5, human platelet alloantigen system 5, integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor), platelet antigen Br, platelet glycoprotein GPIa, platelet membrane glycoprotein Ia, very late activation protein 2 receptor, alpha-2 subunit, very late activation receptor alpha-2 subunit, very late activation receptor subunit alpha-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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