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Western Blot analysis of A431 cell using ITGA2 Polyclonal Antibody at dilution of 1:300
Western Blot analysis of A431 cell using ITGA2 Polyclonal Antibody at dilution of 1:300
Western Blot analysis of A431 cell using ITGA2 Polyclonal Antibody at dilution of 1:300

ITGA2 Polyclonal Antibody

E-AB-15930
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ITGA2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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