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ITGAX Antibody

CSB-PA011879GA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetITGAX
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGAX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3687
  • Target name
    ITGAX
  • Target description
    integrin subunit alpha X
  • Target synonyms
    CD11C, SLEB6, integrin alpha-X, CD11 antigen-like family member C, complement component 3 receptor 4 subunit, integrin alpha X, integrin, alpha X (antigen CD11C (p150), alpha polypeptide), integrin, alpha X (complement component 3 receptor 4 subunit), leu M5, alpha subunit, leukocyte adhesion glycoprotein p150,95 alpha chain, leukocyte adhesion receptor p150,95, leukocyte surface antigen p150,95, alpha subunit, myeloid membrane antigen, alpha subunit, p150 95 integrin alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20702
  • Protein Name
    Integrin alpha-X
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD11c using anti-CD11c antibody (A00357-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysate, Lane 2: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11c antigen affinity purified polyclonal antibody (Catalog # A00357-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11c at approximately 150KD. The expected band size for CD11c is at 128KD.
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