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ITLN1 / Omentin Antibody

LS-C405092
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetITLN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITLN1 / Omentin Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55600
  • Target name
    ITLN1
  • Target description
    intelectin 1
  • Target synonyms
    HL-1, HL1, INTL, ITLN, LFR, hIntL, omentin, intelectin-1, ITLN-1, endothelial lectin HL-1, galactofuranose-binding lectin, intelectin 1 (galactofuranose binding), intestinal lactoferrin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITLN1 using anti-ITLN1 antibody (PB10004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITLN1 antigen affinity purified polyclonal antibody (Catalog # PB10004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITLN1 at approximately 43 kDa. The expected band size for ITLN1 is at 35 kDa.
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