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ProductsBack/JAK1 (phospho Tyr1022/1023) antibody
The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670
The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670
The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670

JAK1 (phospho Tyr1022/1023) antibody

GTX25493
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetJAK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    JAK1 (phospho Tyr1022/1023) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3716
  • Target name
    JAK1
  • Target description
    Janus kinase 1
  • Target synonyms
    AIIDE, JAK1A, JAK1B, JTK3, tyrosine-protein kinase JAK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23458
  • Protein Name
    Tyrosine-protein kinase JAK1
  • Scientific Description
    This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Di Giorgio C, Marchianò S, Marino E, et al. Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis. Front Oncol. 2022,12:939969. doi: 10.3389/fonc.2022.939969
    Read this paper
  • Shen M, Xu Z, Xu W, et al. Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway. J Exp Clin Cancer Res. 2019,38(1):149. doi: 10.1186/s13046-019-1161-8
    Read this paper

Datasheet

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Figure 1. Western blot analysis of JAK1 using anti-JAK1 antibody (A00330). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK1 antigen affinity purified polyclonal antibody (Catalog # A00330) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK1 at approximately 133KD. The expected band size for JAK1 is at 133KD.
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