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Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody

BS-3238R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetJAK1
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Overview

  • Supplier
    Bioss
  • Product Name
    Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3716
  • Target name
    JAK1
  • Target description
    Janus kinase 1
  • Target synonyms
    AIIDE, JAK1A, JAK1B, JTK3, tyrosine-protein kinase JAK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23458
  • Protein Name
    Tyrosine-protein kinase JAK1
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang G, Xu B, Shi F, et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019,2019:7850324. doi: 10.1155/2019/7850324
    Read this paper
  • Huang S, Zhou D, Li YX, et al. In vivo and in vitro effects of microRNA-221 on hepatocellular carcinoma development and progression through the JAK-STAT3 signaling pathway by targeting SOCS3. J Cell Physiol. 2019,234(4):3500-3514. doi: 10.1002/jcp.26863
    Read this paper
  • Iriyama N, Hatta Y, Takei M. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. Cancer Med. 2016,5(11):3223-3234. doi: 10.1002/cam4.925
    Read this paper
  • Wörmann SM, Song L, Ai J, et al. Loss of P53 Function Activates JAK2-STAT3 Signaling to Promote Pancreatic Tumor Growth, Stroma Modification, and Gemcitabine Resistance in Mice and Is Associated With Patient Survival. Gastroenterology. 2016,151(1):180-193.e12. doi: 10.1053/j.gastro.2016.03.010
    Read this paper

Datasheet

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Figure 1. Western blot analysis of JAK1 using anti-JAK1 antibody (A00330). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK1 antigen affinity purified polyclonal antibody (Catalog # A00330) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK1 at approximately 133KD. The expected band size for JAK1 is at 133KD.
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