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[KO Validated] JAK1 Antibody

CAB18323
Assay Genie
TargetJAK1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] JAK1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3716
  • Target name
    JAK1
  • Target description
    Janus kinase 1
  • Target synonyms
    AIIDE, JAK1A, JAK1B, JTK3, tyrosine-protein kinase JAK1
  • Host
    Rabbit
  • Protein IDP23458
  • Protein Name
    Tyrosine-protein kinase JAK1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670
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Figure 1. Western blot analysis of JAK1 using anti-JAK1 antibody (A00330). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK1 antigen affinity purified polyclonal antibody (Catalog # A00330) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK1 at approximately 133KD. The expected band size for JAK1 is at 133KD.
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