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WB analysis of various sample lysates using GTX53992 JAM-A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX53992 JAM-A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX53992 JAM-A antibody. Dilution : 1:1000 Loading : 25microg per lane

JAM-A antibody

GTX53992
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetF11R
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Overview

  • Supplier
    GeneTex
  • Product Name
    JAM-A antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID50848
  • Target name
    F11R
  • Target description
    F11 receptor
  • Target synonyms
    CD321; JAM; JAM1; JAMA; JCAM; junctional adhesion molecule 1; junctional adhesion molecule A; KAT; PAM-1; platelet adhesion molecule 1; platelet F11 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y624
  • Protein Name
    Junctional adhesion molecule A
  • Scientific Description
    Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5 alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (Catalog # A02068-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 41KD. The expected band size for JAM-A/F11R is at 41KD.
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