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ProductsBack/Kallistatin antibody [10N8]

Kallistatin antibody [10N8]

GTX52994
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSERPINA4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Kallistatin antibody [10N8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10N8
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5267
  • Target name
    SERPINA4
  • Target description
    serpin family A member 4
  • Target synonyms
    KAL, KLST, KST, PI-4, PI4, kallistatin, kallistatin, kallikrein inhibitor, peptidase inhibitor 4, protease inhibitor 4 (kallistatin), serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, serpin A4, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, sserpin family A member 4, testicular secretory protein Li 22
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29622
  • Protein Name
    Kallistatin
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINA4 using anti-SERPINA4 antibody (A08426). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA4 antigen affinity purified polyclonal antibody (Catalog # A08426) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA4 at approximately 49KD. The expected band size for SERPINA4 is at 49KD.
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