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Western Blot analysis of Hela cells using GCN5 Polyclonal Antibody
Western Blot analysis of Hela cells using GCN5 Polyclonal Antibody
Western Blot analysis of Hela cells using GCN5 Polyclonal Antibody

KAT2A Antibody

CSB-PA002657
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKAT2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    KAT2A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2648
  • Target name
    KAT2A
  • Target description
    lysine acetyltransferase 2A
  • Target synonyms
    GCN5, GCN5L2, PCAF-b, hGCN5, histone acetyltransferase KAT2A, GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2, General control of amino acid synthesis, yeast, homolog-like 2, K(lysine) acetyltransferase 2A, STAF97, general control of amino acid synthesis protein 5-like 2, histone acetyltransferase GCN5, histone glutaryltransferase KAT2A, histone succinyltransferase KAT2A, hsGCN5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92830
  • Protein Name
    Histone acetyltransferase KAT2A
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KAT2A/GCN5 using anti-KAT2A/GCN5 antibody (PB9713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: 22RV1 Whole Cell Lysate, Lane 3: COLO320 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT2A/GCN5 antigen affinity purified polyclonal antibody (Catalog # PB9713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT2A/GCN5 at approximately 94 kDa. The expected band size for KAT2A/GCN5 is at 94 kDa.
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