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KAT2A Antibody (YA2900, Rabbit)

HY-P83155
MedChem Express
TargetKAT2A
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KAT2A Antibody (YA2900, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2648
  • Target name
    KAT2A
  • Target description
    lysine acetyltransferase 2A
  • Target synonyms
    GCN5, GCN5L2, PCAF-b, hGCN5, histone acetyltransferase KAT2A, GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2, General control of amino acid synthesis, yeast, homolog-like 2, K(lysine) acetyltransferase 2A, STAF97, general control of amino acid synthesis protein 5-like 2, histone acetyltransferase GCN5, histone glutaryltransferase KAT2A, histone succinyltransferase KAT2A, hsGCN5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KAT2A Antibody (YA2900) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2900), targeting KAT2A, with a predicted molecular weight of 94 kDa (observed band size: 94 kDa). KAT2A Antibody (YA2900) can be used for WB, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KAT2A/GCN5 using anti-KAT2A/GCN5 antibody (PB9713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: 22RV1 Whole Cell Lysate, Lane 3: COLO320 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT2A/GCN5 antigen affinity purified polyclonal antibody (Catalog # PB9713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT2A/GCN5 at approximately 94 kDa. The expected band size for KAT2A/GCN5 is at 94 kDa.
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