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KCNJ8 Antibody

CSB-PA012060GA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNJ8
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNJ8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3764
  • Target name
    KCNJ8
  • Target description
    potassium inwardly rectifying channel subfamily J member 8
  • Target synonyms
    KIR6.1, uKATP-1, ATP-sensitive inward rectifier potassium channel 8, inward rectifier K(+) channel Kir6.1, inwardly rectifying potassium channel KIR6.1, potassium channel, inwardly rectifying subfamily J member 8, potassium voltage-gated channel subfamily J member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15842
  • Protein Name
    ATP-sensitive inward rectifier potassium channel 8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kir6.1/KCNJ8 antigen affinity purified polyclonal antibody (Catalog # A04950-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kir6.1/KCNJ8 at approximately 48KD. The expected band size for Kir6.1/KCNJ8 is at 48KD.
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ReactivityHuman, Mouse, Rat
TargetKCNJ8
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TargetKCNJ8
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