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KCNN4 Antibody (YA3533, Mouse)

HY-P83836
MedChem Express
TargetKCNN4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KCNN4 Antibody (YA3533, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3783
  • Target name
    KCNN4
  • Target description
    potassium calcium-activated channel subfamily N member 4
  • Target synonyms
    DHS2, IK, IK1, IKCA1, KCA4, KCa3.1, SK4, SKCa4, hIK1, hIKCa1, hKCa4, hSK4, intermediate conductance calcium-activated potassium channel protein 4, gardos channel, intermediate-conductance Ca2+-activated K+ channel, KCa3.1, potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4, putative Gardos channel, putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel, small conductance calcium-activated potassium channel 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    KCNN4 Antibody (YA3533) is a mouse-derived and non-conjugated IgG1 antibody, targeting to KCNN4. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human PC-3 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
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