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Formalin-fixed and paraffin embedded rat brain labeled with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

KCNN4 Polyclonal Antibody

BS-6675R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNN4
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Overview

  • Supplier
    Bioss
  • Product Name
    KCNN4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3783
  • Target name
    KCNN4
  • Target description
    potassium calcium-activated channel subfamily N member 4
  • Target synonyms
    DHS2, IK, IK1, IKCA1, KCA4, KCa3.1, SK4, SKCa4, hIK1, hIKCa1, hKCa4, hSK4, intermediate conductance calcium-activated potassium channel protein 4, gardos channel, intermediate-conductance Ca2+-activated K+ channel, KCa3.1, potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4, putative Gardos channel, putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel, small conductance calcium-activated potassium channel 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15554
  • Protein Name
    Intermediate conductance calcium-activated potassium channel protein 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Comparing Effects of Transforming Growth Factor beta1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels. Lively S et al., 2018, Front Cell Neurosci
    Read this paper
  • Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells. Zhang P et al., 2016, PLoS One
    Read this paper

Datasheet

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Figure 1. Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human PC-3 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
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