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Anti-KCNN4 / KCa3.1 (N-term) Antibody

119-12613
RayBiotech
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKCNN4
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Rabbit Anti-Human KCNN4 / KCa3.1 (N-Terminus)
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Gene ID3783
  • Target name
    KCNN4
  • Target description
    potassium calcium-activated channel subfamily N member 4
  • Target synonyms
    DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; intermediate conductance calcium-activated potassium channel protein 4; intermediate-conductance Ca2+-activated K+ channel, KCa3.1; KCa3.1; KCA4; potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel; putative Gardos channel; SK4; SKCa 4; SKCa4; small conductance calcium-activated potassium channel 4
  • Host
    Rabbit
  • Protein IDO15554
  • Protein Name
    Intermediate conductance calcium-activated potassium channel protein 4
  • Scientific Description
    KCNN4 / KCa3.1 Rabbit anti-Human Polyclonal (N-Terminus) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human PC-3 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
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