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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

KDM6A antibody [N2C1], Internal

GTX121246
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetKDM6A
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Overview

  • Supplier
    GeneTex
  • Product Name
    KDM6A antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.68 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7403
  • Target name
    KDM6A
  • Target description
    lysine demethylase 6A
  • Target synonyms
    KABUK2, UTX, bA386N14.2, lysine-specific demethylase 6A, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)), histone demethylase UTX, lysine (K)-specific demethylase 6A, ubiquitously transcribed tetratricopeptide repeat protein X-linked, ubiquitously-transcribed TPR gene on the X chromosome
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15550
  • Protein Name
    Lysine-specific demethylase 6A
  • Scientific Description
    This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Luo D, Chen M, Li Q, et al. CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Exp Hematol Oncol. 2023,12(1):77. doi: 10.1186/s40164-023-00440-z
    Read this paper
  • Sun W, Lee KL, Poellinger L, et al. Catalytic domain-dependent and -independent transcriptional activities of the tumour suppressor histone H3K27 demethylase UTX/KDM6A in specific cancer types. Epigenetics. 2023,18(1):2222245. doi: 10.1080/15592294.2023.2222245
    Read this paper
  • Kitajima S, Sun W, Lee KL, et al. A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition. Sci Rep. 2021,11(1):4538. doi: 10.1038/s41598-021-83857-y
    Read this paper
  • Davis EJ, Broestl L, Abdulai-Saiku S, et al. A second X chromosome contributes to resilience in a mouse model of Alzheimer's disease. Sci Transl Med. 2020,12(558). doi: 10.1126/scitranslmed.aaz5677
    Read this paper
  • Tang QY, Zhang SF, Dai SK, et al. UTX Regulates Human Neural Differentiation and Dendritic Morphology by Resolving Bivalent Promoters. Stem Cell Reports. 2020,15(2):439-453. doi: 10.1016/j.stemcr.2020.06.015
    Read this paper
  • Imuta H, Fujita D, Oba S, et al. Histone methylation and demethylation are implicated in the transient and sustained activation of the interleukin-1β gene in murine macrophages. Heart Vessels. 2020,35(12):1746-1754. doi: 10.1007/s00380-020-01670-5
    Read this paper
  • Perrigue PM, Rakoczy M, Pawlicka KP, et al. Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts. Cancers (Basel). 2020,12(7). doi: 10.3390/cancers12071745
    Read this paper
  • Kato H, Asamitsu K, Sun W, et al. Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization. Oncogene. 2020,39(16):3322-3335. doi: 10.1038/s41388-020-1218-3
    Read this paper
  • Gozdecka M, Meduri E, Mazan M, et al. UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs. Nat Genet. 2018,50(6):883-894. doi: 10.1038/s41588-018-0114-z
    Read this paper
  • Zhang H, Fillmore Brainson C, Koyama S, et al. Lkb1 inactivation drives lung cancer lineage switching governed by Polycomb Repressive Complex 2. Nat Commun. 2017,8:14922. doi: 10.1038/ncomms14922
    Read this paper

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Figure 1. Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody (A01286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody (Catalog # A01286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UTX/KDM6A at approximately 154 kDa. The expected band size for UTX/KDM6A is at 154 kDa.
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