Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![KEAP1 antibody [C1C3] detects KEAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX106142/GTX106142_39897_42403_IFA_w_23060120_177.webp "KEAP1 antibody [C1C3] detects KEAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500. Blue: Hoechst 33342 staining.")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
KEAP1 antibody [C1C3]
GTX106142
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetKEAP1
Overview
- SupplierGeneTex
- Product NameKEAP1 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9817
- Target nameKEAP1
- Target descriptionkelch like ECH associated protein 1
- Target synonymsINrf2, KLHL19, kelch-like ECH-associated protein 1, KEAP1 delta C, cytosolic inhibitor of Nrf2, kelch-like family member 19, kelch-like protein 19
- HostRabbit
- IsotypeIgG
- Protein IDQ14145
- Protein NameKelch-like ECH-associated protein 1
- Scientific DescriptionThis gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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