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Keap1 Polyclonal Antibody

CAC07039
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetKEAP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Keap1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9817
  • Target name
    KEAP1
  • Target description
    kelch like ECH associated protein 1
  • Target synonyms
    INrf2, KLHL19, kelch-like ECH-associated protein 1, KEAP1 delta C, cytosolic inhibitor of Nrf2, kelch-like family member 19, kelch-like protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14145
  • Protein Name
    Kelch-like ECH-associated protein 1
  • Scientific Description
    The Keap1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IF analysis of KEAP1 using anti-KEAP1 antibody (A00514-3). KEAP1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-KEAP1 Antibody (A00514-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KEAP1 Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, DAB staining.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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