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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KEAP1 Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KEAP1 Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KEAP1 Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, DAB staining.

KEAP1 Polyclonal Antibody

BS-3648R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKEAP1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    KEAP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9817
  • Target name
    KEAP1
  • Target description
    kelch like ECH associated protein 1
  • Target synonyms
    cytosolic inhibitor of Nrf2; INrf2; KEAP1 delta C; kelch-like ECH-associated protein 1; kelch-like family member 19; kelch-like protein 19; KLHL19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14145
  • Protein Name
    Kelch-like ECH-associated protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Lycopene Prevents DEHP-Induced Leydig Cell Damage with the Nrf2 Antioxidant Signaling Pathway in Mice. Zhao Y et al., 2020 Feb 19, J Agric Food Chem
    Read more
  • Effect of acrylamide on BEAS-2B normal human lung cells: Cytotoxic, oxidative, apoptotic and morphometric analysis. Kacar S et al., 2019 Jul, Acta Histochem
    Read more
  • Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1. Meng Q et al., 2018, J Anim Sci Biotechnol
    Read more
  • Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Kouam AF et al., 2017, Front Pharmacol
    Read more
  • The electrophilic character of quinones is essential for the suppression of Bach1. Su C et al., 2017 Jul 15, Toxicology
    Read more
  • Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia. Chen YH et al., 2016, PLoS One
    Read more
  • Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation but not Keap1-Cullin3 complex dissociation. Su C et al., 2015 Apr 20, Chem Res Toxicol
    Read more

Datasheet

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Figure 2. IF analysis of KEAP1 using anti-KEAP1 antibody (A00514-3). KEAP1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-KEAP1 Antibody (A00514-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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