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Western blot All lanes: KEAP1 antibody at 2microg/ml Lane 1: Mouse kidney tissue Lane 2: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa
Western blot All lanes: KEAP1 antibody at 2microg/ml Lane 1: Mouse kidney tissue Lane 2: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa
Western blot All lanes: KEAP1 antibody at 2microg/ml Lane 1: Mouse kidney tissue Lane 2: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa

KEAP1 Antibody

CSB-PA012147LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKEAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KEAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9817
  • Target name
    KEAP1
  • Target description
    kelch like ECH associated protein 1
  • Target synonyms
    INrf2, KLHL19, kelch-like ECH-associated protein 1, KEAP1 delta C, cytosolic inhibitor of Nrf2, kelch-like family member 19, kelch-like protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14145
  • Protein Name
    Kelch-like ECH-associated protein 1
  • Scientific Description
    Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IF analysis of KEAP1 using anti-KEAP1 antibody (A00514-3). KEAP1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-KEAP1 Antibody (A00514-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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