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Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.

Ki-67 Polyclonal Antibody

BS-2130R
Bioss
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMKI67
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Overview

  • Supplier
    Bioss
  • Product Name
    Ki-67 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4288
  • Target name
    MKI67
  • Target description
    marker of proliferation Ki-67
  • Target synonyms
    KIA, MIB-, MIB-1, PPP1R105, proliferation marker protein Ki-67, Molecular Immunology Borstel antibody 1, antigen Ki67, antigen identified by monoclonal antibody Ki-67, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46013
  • Protein Name
    Proliferation marker protein Ki-67
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma. Hou Y et al., 2019 Dec 5, Cell Death Dis
    Read this paper
  • Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Li R et al., 2020 Jan, Biofactors
    Read this paper
  • Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Liu F et al., 2019 Dec, Biomed Pharmacother
    Read this paper
  • Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway. Li T et al., 2019 Jul 17, Molecules
    Read this paper
  • LncRNA differentiation antagonizing non-protein coding RNA promotes proliferation and invasion through regulating miR-135a/NLRP37 axis in pancreatic cancer. Tang Y et al., 2019 Jul 3, Invest New Drugs
    Read this paper
  • LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer. Wang A et al., 2019 Feb 8, Invest New Drugs
    Read this paper
  • LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR. Luo M et al., 2018 Nov 18, J Cell Biochem
    Read this paper
  • A retrospective, single-center cohort study on 65 patients with primary retroperitoneal liposarcoma. Wu YX et al., 2018 Feb, Oncol Lett
    Read this paper
  • Silencing of CEMIP suppresses Wnt/beta-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells. Liang G et al., 2018 Jan, Acta Histochem
    Read this paper
  • Tumor pH-Responsive Release of Drug-Conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy. He N et al., 2017 Sep 27, ACS Appl Mater Interfaces
    Read this paper

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Figure 1. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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