Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Ki-67 Polyclonal Antibody
BS-2130R
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMKI67
Overview
- SupplierBioss
- Product NameKi-67 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierIHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID4288
- Target nameMKI67
- Target descriptionmarker of proliferation Ki-67
- Target synonymsKIA, MIB-, MIB-1, PPP1R105, proliferation marker protein Ki-67, Molecular Immunology Borstel antibody 1, antigen Ki67, antigen identified by monoclonal antibody Ki-67, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105
- HostRabbit
- IsotypeIgG
- Protein IDP46013
- Protein NameProliferation marker protein Ki-67
- Storage Instruction-20°C
- UNSPSC12352203
References
- Hou Y, Wang Z, Huang S, et al. SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma. Cell Death Dis. 2019,10(12):929. doi: 10.1038/s41419-019-2163-3Read this paper
- Li R, Song Y, Ji Z, et al. Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Biofactors. 2020,46(1):158-167. doi: 10.1002/biof.1583Read this paper
- Liu F, Wang L, Yi S, et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019,120:109533. doi: 10.1016/j.biopha.2019.109533Read this paper
- Li T, Wei Z, Sun Y, et al. Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway. Molecules. 2019,24(14). doi: 10.3390/molecules24142596Read this paper
- Tang Y, Cao G, Zhao G, et al. LncRNA differentiation antagonizing non-protein coding RNA promotes proliferation and invasion through regulating miR-135a/NLRP37 axis in pancreatic cancer. Invest New Drugs. 2020,38(3):714-721. doi: 10.1007/s10637-019-00798-0Read this paper
- Wang A, Zhao C, Gao Y, et al. LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer. Invest New Drugs. 2019,37(6):1127-1134. doi: 10.1007/s10637-018-00721-zRead this paper
- Luo M, Kong D, Pei D, et al. LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR. J Cell Biochem. 2019,120(5):7506-7515. doi: 10.1002/jcb.28025Read this paper
- Wu YX, Liu JY, Liu JJ, et al. A retrospective, single-center cohort study on 65 patients with primary retroperitoneal liposarcoma. Oncol Lett. 2018,15(2):1799-1810. doi: 10.3892/ol.2017.7533Read this paper
- Liang G, Fang X, Yang Y, et al. Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells. Acta Histochem. 2018,120(1):56-63. doi: 10.1016/j.acthis.2017.11.002Read this paper
- He N, Chen Z, Yuan J, et al. Tumor pH-Responsive Release of Drug-Conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy. ACS Appl Mater Interfaces. 2017,9(38):32534-32544. doi: 10.1021/acsami.7b09519Read this paper
Datasheet
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