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ProductsBack/Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: OTI3D11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MKI67 fragment (N- and C-terminus) (Cat# RC220910, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKI67 (Cat# TA500265).
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MKI67 fragment (N- and C-terminus) (Cat# RC220910, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKI67 (Cat# TA500265).
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MKI67 fragment (N- and C-terminus) (Cat# RC220910, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKI67 (Cat# TA500265).

Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CF500265
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMKI67
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: OTI3D11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3D11
  • Gene ID4288
  • Target name
    MKI67
  • Target description
    marker of proliferation Ki-67
  • Target synonyms
    KIA, MIB-, MIB-1, PPP1R105, proliferation marker protein Ki-67, Molecular Immunology Borstel antibody 1, antigen Ki67, antigen identified by monoclonal antibody Ki-67, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP46013
  • Protein Name
    Proliferation marker protein Ki-67
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MKI67 mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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