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Klf13 Polyclonal Antibody

CAC11428
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKLF13
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Overview

  • Supplier
    Biomatik
  • Product Name
    Klf13 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51621
  • Target name
    KLF13
  • Target description
    KLF transcription factor 13
  • Target synonyms
    BTEB3, FKLF2, NSLP1, RFLAT-1, RFLAT1, Krueppel-like factor 13, BTE-binding protein 3, Kruppel like factor 13, RANTES factor of late activated T lymphocytes-1, basic transcription element binding protein 3, novel Sp1-like zinc finger transcription factor 1, transcription factor BTEB3, transcription factor NSLP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2Y9
  • Protein Name
    Krueppel-like factor 13
  • Scientific Description
    The Klf13 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLF13 using anti-KLF13 antibody (A07076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF13 antigen affinity purified polyclonal antibody (Catalog # A07076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF13 at approximately 38 kDa. The expected band size for KLF13 is at 31 kDa.
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