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Western Blot analysis of L929 cells using KLF13 Polyclonal Antibody
Western Blot analysis of L929 cells using KLF13 Polyclonal Antibody
Western Blot analysis of L929 cells using KLF13 Polyclonal Antibody

KLF13 Antibody

CSB-PA005502
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetKLF13
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Overview

  • Supplier
    Cusabio
  • Product Name
    KLF13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51621
  • Target name
    KLF13
  • Target description
    KLF transcription factor 13
  • Target synonyms
    BTEB3, FKLF2, NSLP1, RFLAT-1, RFLAT1, Krueppel-like factor 13, BTE-binding protein 3, Kruppel like factor 13, RANTES factor of late activated T lymphocytes-1, basic transcription element binding protein 3, novel Sp1-like zinc finger transcription factor 1, transcription factor BTEB3, transcription factor NSLP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2Y9
  • Protein Name
    Krueppel-like factor 13
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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WB analysis of various samples using GTX66827 KLF13 (acetyl lys166) antibody. Lane1 : Hela whole cell lysate(40ug) Lane2 : Jurkat whole cell lysate(40ug) Lane3 : NIH-3T3 whole cell lysate(40ug) Lane4 : PC12 whole cell lysate(40ug) Dilution : 1:500
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Figure 1. Western blot analysis of KLF13 using anti-KLF13 antibody (A07076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF13 antigen affinity purified polyclonal antibody (Catalog # A07076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF13 at approximately 38 kDa. The expected band size for KLF13 is at 31 kDa.
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