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[KO Validated] ZMYND8 Antibody

CAB8737
Assay Genie
TargetZMYND8
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] ZMYND8 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID23613
  • Target name
    ZMYND8
  • Target description
    zinc finger MYND-type containing 8
  • Target synonyms
    PRKCBP1, PRO2893, RACK7, MYND-type zinc finger-containing chromatin reader ZMYND8, CTCL tumor antigen se14-3, RACK like clone 7, cutaneous T-cell lymphoma-associated antigen se14-3, predicted protein of HQ2893, protein kinase C-binding protein 1, transcription coregulator ZMYND8, zinc finger MYND domain-containing protein 8
  • Host
    Rabbit
  • Protein IDQ9ULU4
  • Protein Name
    MYND-type zinc finger-containing chromatin reader ZMYND8
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Hela cell lysates; Lane 2: ZMYND8 knockout (KO) HeLa cell lysates probed with ZMYND8 Polyclonal Antibody, Unconjugated (bs-55227R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYND8 antigen affinity purified polyclonal antibody (Catalog # A06830-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYND8 at approximately 150 kDa. The expected band size for ZMYND8 is at 132 kDa.
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