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ProductsBack/ZMYND8 / RACK7 Antibody

ZMYND8 / RACK7 Antibody

LS-C410271
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetZMYND8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ZMYND8 / RACK7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 66kDa/82kDa/109-138kDa, while the observed MW by Western blot was 165kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 66kDa/82kDa/109-138kDa, while the observed MW by Western blot was 165kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23613
  • Target name
    ZMYND8
  • Target description
    zinc finger MYND-type containing 8
  • Target synonyms
    PRKCBP1, PRO2893, RACK7, MYND-type zinc finger-containing chromatin reader ZMYND8, CTCL tumor antigen se14-3, RACK like clone 7, cutaneous T-cell lymphoma-associated antigen se14-3, predicted protein of HQ2893, protein kinase C-binding protein 1, transcription coregulator ZMYND8, zinc finger MYND domain-containing protein 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYND8 antigen affinity purified polyclonal antibody (Catalog # A06830-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYND8 at approximately 150 kDa. The expected band size for ZMYND8 is at 132 kDa.
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